Peter's urease resources


I would like to acknowledge the European Community TMR program (for the funding of my PhD grant) and the UK Ministry of Agriculture, Fisheries and Food and the Scottish Executive Rural Affairs Department for financial support. Many thanks go to my PhD supervisors, Prof. Dr. Howard Davies and Dr Mark Taylor for their often very helpful scientific advise.

Urease related protocols

Detection of urease after native polyacrylamide gel electrophoresis
Plant urease extraction and quantification
Extraction and detection of urea after foliar sprays

Some results

Urease detection in various plants with in-gel staining
gels
Urease activity in organs of field-grown potato
activity plots
Heat stability of urease in extracts from potato leaves
activity plots
Plant urease detection with commercial anti jackbean-urease antibodies
westerns

Alignments

Annotated alignment of potato urease genomic clones
pdf-file, 75k
Alignment of urease protein sequences from eukaryotes and prokaryotes (obtained with ClustalW) and corresponding sequences in fasta format
alignment; pdf-file, 177k
fasta sequences, ASCII-file 38k

Researchers working on urease

Robert P. Hausinger
Urease and urease accessory proteins from Klebsiella aerogenes

Harry Mobley
Urease and urease accessory proteins from Proteus mirabilis and Helicobacter pylori

Joseph C. Polacco
Urease and urease accessory proteins in plants (soybean)

Urease links

Urease in the Worthington Enzyme Manual
Some general information (a bit old), containing a protocol for a coupled enzyme assay for urease using glutamate dehydrogenase

Urease in HOMSTRAD
HOMSTRAD (HOMologous STRucture Alignment Database) is a curated database of structure-based alignments for homologous protein families. The alpha subunits of ureases from Klebsialla aerogenes and Bacillus pasteurii are aligned. The structure-based alignment can be viewed in parallel to an alignment of other urease sequences for which structural data is not available. Links to the urease-relevant sites in PROSITE and PFAM are provided.

Determination of soil urease activity
A simple method to determine soil urease activity

Klebsiella aerogenes urease home page
A page with lots of background information to urease and urease accessory proteins from Klebsiella aerogenes. Contains also a 3D model of urease (you need Chime plug-in).

Peter's CV and personal stuff

Peter's curriculum vitae
PDF file, 21k
MS Word 97 file, 100k


Claus-Peter Witte, last updated in July 2001 B
Email: cpwitte@hotmail.com